PROTEIN AGGREGATION

Nucleation-elongation · Avrami equation · fibril growth · ThT fluorescence · inhibitors
Nucleation-elongation
Lag phase → exponential → plateau

Avrami equation
θ(t) = 1 − exp(−(kt)ⁿ)
n=1: 1D seeded growth
n=2: 2D, n=3: 3D nucleation

Seeding: eliminates lag phase
Inhibitor: shifts curve right,
reduces plateau

ThT fluorescence ∝ fibril mass
F(t) ≈ F_max·θ(t)

t₁/₂ ∝ kₙ^(-1/2)·k₊^(-1)