The FFL: how gene networks achieve pulse generation, delay, and sign-sensitive filtering
In a FFL, transcription factor X activates Y; both X and Y regulate output Z. The sign of each edge determines function.
Coherent FFL (C1): X→Y→Z, X→Z (all +). Acts as a persistence detector — delays activation, fast off.
Incoherent FFL (I1): X→Y→Z but X⊣Z. Generates a pulse, acts as fold-change detector.