Single-molecule MM
State: free (E) or bound (ES)
Switch rates: kon·[S] and koff+kcat
Burst statistics
ON-time ~ Exp(koff+kcat)
OFF-time ~ Exp(kon·[S])
⟨ton⟩=1/(koff+kcat)
⟨toff⟩=1/(kon·[S])
FRET: E=R₀⁶/(R₀⁶+r⁶)
high E = donor near acceptor
(bound state shows higher E)
State: free (E) or bound (ES)
Switch rates: kon·[S] and koff+kcat
Burst statistics
ON-time ~ Exp(koff+kcat)
OFF-time ~ Exp(kon·[S])
⟨ton⟩=1/(koff+kcat)
⟨toff⟩=1/(kon·[S])
FRET: E=R₀⁶/(R₀⁶+r⁶)
high E = donor near acceptor
(bound state shows higher E)