Steady-state flux distribution in a toy central carbon metabolism network
Flux Balance Analysis (FBA): maximize biomass production subject to stoichiometric constraints Sv=0 and flux bounds. This toy network models glycolysis → TCA cycle → OXPHOS with ATP/NADH balancing. Edge thickness = flux magnitude; color: blue=glycolysis, green=TCA, orange=biosynthesis, red=fermentation. Overflow metabolism (Warburg effect): if O₂ is limiting, carbon overflows to fermentation.