Metabolic Flux Analysis — Steady-State Stoichiometry
Flux balance in metabolic networks — stoichiometric constraints, nullspace, and optimal growth
Controls
Glucose uptake v₁ (max):
10
O₂ uptake v₂ (max):
10
ATP demand v_atp:
5
Objective:
Maximize growth
Maximize ATP
Minimize waste
Solve FBA
Steady-state constraint:
S·v = 0 (stoichiometry)
v_min ≤ v ≤ v_max
FBA: max c·v s.t. S·v=0, bounds
Flux cone = {v: S·v=0, v≥0}
Extreme rays = elementary flux modes