ENZYME KINETICS & INHIBITION

Michaelis-Menten · competitive/uncompetitive/mixed inhibition · Hill cooperativity
MM equation
v = Vmax·Sⁿ/(Km_app^n+Sⁿ)

Competitive: Km↑, Vmax same
Uncompetitive: both↓
Mixed: Km and Vmax both change

Hill n>1 → positive cooperativity
Hill plot: log(v/(Vmax-v)) vs log[S]
slope = n (Hill coefficient)